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General Information
Symbol
P{E(spl)m8-HLH-2.61}
FlyBase ID
FBtp0004163
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
E(spl)m8-lacZ, E(spl)m8 2.61-lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Component allele Ecol\lacZE(spl)m8-HLH.2.61
Product class / Tool use(s)
Encoded product / tool
Description

2.612kb of 5' E(spl)m8-HLH promoter sequences, driving expression of lacZ from a minimal Hsp70 promoter.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Ecol\lacZE(spl)m8-HLH.2.61 drives expression in the outer optic anlage, with stronger expression near the medial transition zone where the medulla neuroblasts form.

E(spl)m8-HLH transcript is expressed in the inner and outer optic anlagen (IPC, OPC), most strongly in the medial epithelial cells of the OPC. Expression is also observed in medulla forming neuroblasts, and in newly generated medulla neurons and their axons.

In third instar imaginal discs, expression initially strong in regions undergoing PNS development; in the leg, these correspond to the chordotonal organs in the femur and tibia. Expression is observed near the presumptive joints of the leg; resolves in pupa into one-cell wide stripes proximal to the leg constrictions.

In third instar wing disc, pattern of expression described as 'resembling' that of E(spl).

In early embryo, expression differs slightly from that of E(spl) : expression in the mesectoderm anlagen is in a pair-rule pattern rather than continuous, and expression extends into the mesoderm anlagen rather than being restricted to the mesectoderm anlagen. In stage 9 embryos, expressed in a ladder-like pattern of cells in the ventral neurectoderm.

 
Marker for
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Secondary FlyBase IDs
    References (18)