FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{MSE-lacZ}
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General Information
Symbol
P{MSE-lacZ}
FlyBase ID
FBtp0004188
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZeve.MSE
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZeve.MSE
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

The 480bp eve minimal stripe 2 enhancer (MSE) (sequence from -1.55kb to -1.1kb upstream of the eve transcription start site) is fused upstream of eve basal promoter sequence from -42bp, the 100bp eve leader sequence and the first 22 codons of eve protein coding sequence, fused to the lacZ coding sequence at codon 5.

(Small et al., 1992)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5
organism | anterior | restricted

Comment: anterior stripe

(Jiang et al., 1992)
parasegment 3

Comment: eve stripe 2

(Kirov et al., 1994)
organism | anterior
(Small et al., 1992)
Additional Information
Statement
Reference

In early embryo, high levels of expression in region corresponding to the second stripe of the wild-type eve expression pattern; very low levels of expression outside the eve stripe 2 region.

(Jiang et al., 1992)

In early embryos, expression initially detected in a broad region that spans nearly the entire anterior half of the embryo; after a short time, refined into a stripe. Shown to correspond to the second eve stripe, with some quantitative differences in expression along the dorsal-ventral axis.

(Small et al., 1992)
 
Marker for
Reflects expression of
eve
(Kirov et al., 1994, Jiang et al., 1992, Small et al., 1992)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
MSE
(Muller et al., 2003)
P{MSE-lacZ}
(Wang et al., 2010, FlyBase, 1996-)
eve 2-lacZ
(Struffi et al., 2011)
eve2-lacZ
(Andrioli et al., 2002)
Secondary FlyBase IDs
    References (14)