FB2026_02 , released June 18, 2026
Recombinant Construct: P{-1200LACcTm-3'}
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General Information
Symbol
P{-1200LACcTm-3'}
FlyBase ID
FBtp0004501
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZTm1.1200.cTm
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZTm1.1200.cTm
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

lacZ gene expression is driven by a promoter and 5' leader sequence 1.2kb fragment of Tm1 gene from intron 3 and exon 4 and the 3' untranslated sequence (3'UTR) of Tm1.

(Hales et al., 1994)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4
embryo | posterior
(Hales et al., 1994)
embryonic stage 5
embryonic cortex
(Hales et al., 1994)
embryonic stage 6 -- 11
germ band
(Hales et al., 1994)
cephalic furrow
(Hales et al., 1994)
ventral furrow
(Hales et al., 1994)
embryonic stage 6 -- 15
posterior midgut primordium
(Hales et al., 1994)
anterior midgut primordium
(Hales et al., 1994)
embryonic stage 11
ectoderm | segmentally repeated
(Hales et al., 1994)
embryonic stage 13
dorsal ectoderm
(Hales et al., 1994)
embryonic stage 13 -- 17
embryonic midgut constriction
(Hales et al., 1994)
presumptive embryonic/larval central nervous system
(Hales et al., 1994)
embryonic brain
(Hales et al., 1994)
adult stage & oogenesis
oocyte
(Hales et al., 1994)
nurse cell
(Hales et al., 1994)
Additional Information
Statement
Reference

In this construct, EcollacZ is sandwiched between Tm1 sequences from -1200 bp to +240 bp (relative to start of transcription), and Tm1 3'UTR sequence. EcollacZ expression is driven in a pattern resembling that of endogenous Tm1. Ovaries, nurse cells and subsequently, oocytes, express EcollacZ. Follicle cells, however, do not express EcollacZ. Maternal expression is detected in early embryogenesis. Subsequently, expression is detected in the cephalic and ventral furrows, and the germ band. The anterior and posterior midgut primordia stain fr EcollacZ. At stage 11, staining is detected in a segmentally repeated pattern in the ectoderm. After stage 13, the brain, CNS and midgut constriction stain for EcollacZ.

(Hales et al., 1994)
 
Marker for
Reflects expression of
Tm1
(Hales et al., 1994)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{-1200LACcTm-3'}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)