FlyBase Recombinant Construct Report: P{apC-tau-lacZ}

General Information

Symbol

P{apC-tau-lacZ}

FlyBase ID

FBtp0004965

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^{ap.C.Tag:MT(btau)}

Also Known As

apC-tau-lacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Ecol\lacZ^{ap.C.Tag:MT(btau)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

ap_apC

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:MT(btau)

microtubule protein localization tag

Description

Sequences from -6kb to -12kb upstream of the ap transcription start site direct expression of a lacZ reporter tagged with Tag:MT(btau).

(Lundgren et al., 1995)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^{ap.C.Tag:MT(btau)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval ventral nerve cord | restricted

(Lundgren et al., 1995)

interneuron of larval ventral nerve cord

(Lundgren et al., 1995)

somatic muscle cell | subset

(Lundgren et al., 1995)

brain | restricted

(Lundgren et al., 1995)

interneuron | subset

(Thor et al., 1999)

interneuron

(Lundgren et al., 1995)

larval stage

wing disc

(Lundgren et al., 1995)

Additional Information

Statement

Reference

Accurately reflects expression of ap in terms of cells in which that gene is expressed; however, the ap protein is nuclear, whereas this reporter is targeted to microtubules and thus labels cell bodies and axons. In embryonic VNC, expresssion restricted to 7 cells (interneurons) in each thoracic hemisphere.

(Lundgren et al., 1995)

Analysis of BtauMAPT^{ap.C.T:EcollacZ}expression suggests that ap-expressing cells are interneurons that startto elongate axons soon after they begin expressing ap protein.

(Lundgren et al., 1995)

Marker for

Reflects expression of

(Thor et al., 1999, Lundgren et al., 1995)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{C4tLZ}

(Lundgren et al., 1995)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Ap-C-tau-lacZ

(Crowner et al., 2002)

ApC Tau lacZ

(Lee et al., 2004)

ApC-tauβgal

(Ratnaparkhi et al., 2002)

P{apC-tau-lacZ}

(FlyBase, 1996-)

apC-tau-lacZ

(Lee et al., 2007, Boyle et al., 2006)

apC-tau-lacZ#2.1

(Miguel-Aliaga et al., 2004)

apC2.1-tau-lacZ

(Stratmann et al., 2019)

apterous-tau-lacZ

(Callahan et al., 1998)

apterous-tau:lacZ

(Layden et al., 2006)

Secondary FlyBase IDs

References (20)

Report Sections

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