Construct: Insertion of an HpaII-XbaI fragment (present in the genomic fragment of Sgs3e.g61), in reverse orientation, into the XbaI site upstream of Sgs3e in Sgs3e.g6. Contains the Sgs3e coding region flanked by 2.06kb of 5' Sgs3e flanking sequence and 0.3kb of 3' Sgs3e flanking sequence. Contains the same genomic DNA as Sgs7For.g61I.
Construct: Contains the same genomic DNA as Sgs3e.g61I. The HpaII-XbaI fragment (which is in reverse orientation) includes 3' flanking region and the most 3' 100bp of Sgs7For transcribed sequences.