Construct: Contains the same genomic DNA as Sgs3e.g61-3'I. The HpaII-XbaI fragment (which is in reverse orientation) contains 3' flanking region and the most 3' 100bp of Sgs7For transcribed sequences.
Construct: Contains the same XbaI-XhoI fragment as Sgs3e.g6. Also contains the HpaII-XbaI fragment present in the Sgs3e.g61 inserted at the XhoI site, downstream of the Sgs3e coding region, in reverse orientation. Contains the same genomic DNA as Sgs7For.g61-3'I.