FlyBase Recombinant Construct Report: P{m8-lacZ}

General Information

Symbol

P{m8-lacZ}

FlyBase ID

FBtp0005425

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^{E(spl)m8-HLH.-1166+87}

Also Known As

E(spl)m8-lacZ, m8-lacZ, E(spl)m8 lacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Ecol\lacZ^{E(spl)m8-HLH.-1166+87}

Transgenic product class

Component

Use(s)

Regulatory region(s)

E(spl)m8-HLH_-1166/+87

Encoded product / tool

lacZ

reporter enzyme

Description

E(spl)m8-HLH promoter fragment extending from -1166 to +87 drives expression of lacZ.

(Lecourtois and Schweisguth, 1995)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^{E(spl)m8-HLH.-1166+87}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8 -- 10

ventral nerve cord primordium

(Lecourtois and Schweisguth, 1995)

embryonic stage 9

stage 1 neuroblast

(Lecourtois and Schweisguth, 1995)

embryonic stage 12

ventral neurectoderm

(Monastirioti et al., 2010)

third instar larval stage

proneural cluster

(Shukla et al., 2017)

wandering third instar larval stage

anterior-posterior compartment boundary of the wing disc

(Klein, 2003)

presumptive wing vein L3

(Klein, 2003)

Additional Information

Statement

Reference

In stage 9 embryo, expressed in two longitudinal rows of ectodermal cells on either side of the ventral midline and in some additional cells located between these two rows; also expressed in delaminating SI neuroblasts.

(Lecourtois and Schweisguth, 1995)

Marker for

proneural cluster

(Shukla et al., 2017)

Reflects expression of

E(spl)m8-HLH

(Shukla et al., 2017, Klein, 2003)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

26786

P{m8-lacZ}1, w¹¹¹⁸

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

E(spl) m8-lacZ

(Lee et al., 2000)

E(spl)-m8-lacZ

(Monastirioti et al., 2010)

E(spl)m8 lacZ

(Klein, 2003, Klein et al., 2000)

E(spl)m8-lacZ

(Shukla et al., 2017, Chou et al., 2015, Ulvklo et al., 2012, Kaspar et al., 2008, Kamimura et al., 2004, Koelzer and Klein, 2003, Negeri et al., 2002)

E(spl)m8::LacZ

(De Joussineau et al., 2003)

HES.m8-LacZ

(Shen et al., 2025)

P{m8-lacZ}

(Mohit et al., 2006, FlyBase, 1996-)

m8-lacZ

(Akiba et al., 2024, Li et al., 2020, Chen et al., 2018, Yamakawa et al., 2012, Kugler and Nagel, 2007, Nagel et al., 2000, Apidianakis et al., 1999)

Secondary FlyBase IDs

References (29)

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