FB2026_02 , released June 18, 2026
Recombinant Construct: P{sβ-324}
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General Information
Symbol
P{sβ-324}
FlyBase ID
FBtp0005476
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZSry-β.-324
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZSry-β.-324
Transgenic product class
Component
Use(s)
Regulatory region(s)
Sry-beta_-324
Encoded product / tool
lacZ
Description

Fragment of Sry-β upstream region from -324 to +137 (where +1 refers to the Sry-β transcription start site) replaces the Sry-δ sequences. The first four amino acids of Sry-β are fused in frame to lacZ.

(Yanicostas et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 12
organism | ubiquitous | uniform
(Yanicostas et al., 1995)
larval stage
(Yanicostas et al., 1995)
third instar larval stage | male
testis | intense
(Yanicostas et al., 1995)
third instar larval stage | female
ovary | intense
(Yanicostas et al., 1995)
third instar larval stage
embryonic/larval fat body
(Yanicostas et al., 1995)
imaginal disc
(Yanicostas et al., 1995)
embryonic/larval salivary gland | intense
(Yanicostas et al., 1995)
adult stage | male
ejaculatory duct
(Yanicostas et al., 1995)
male accessory gland
(Yanicostas et al., 1995)
adult stageoogenesis stage S1 -- S7
germarium
(Yanicostas et al., 1995)
adult stage | femaleoogenesis stage S2 -- S7
nurse cell
(Yanicostas et al., 1995)
adult stageoogenesis stage S7 -- S10
follicle cell
(Yanicostas et al., 1995)
adult stage | femaleoogenesis stage S8 -- S10
follicle cell
(Yanicostas et al., 1995)
spermatogenesis & adult stage
testis | restricted
(Yanicostas et al., 1995)
Additional Information
Statement
Reference

In EcollacZ-reporter constructs driven by either -324 to +137 or the -117 to +137 Sry-β sequence (+1 is the Sry-β transcription start site), expression is detected at high levels in the germarium and nurse cells from stage S1 to S7. Follicle cells begin to stain for EcollacZ at stage S7, and by stage S10, follicle cell staining is strong, while expression is faint in nurse cells and oocytes. In adult males, staining is detected in the male accessory gland, the ejaculatory duct, and in the non-apical portions of the testis (suggestinghat expression begins in late premeiosis). Staining is also seen in larvae, in larval tissue, the gonads of both sexes, and imaginal tissue.

(Yanicostas et al., 1995)
 
Marker for
Reflects expression of
Sry-β
(Yanicostas et al., 1995)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{sβ-324}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)