FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{cutD-hsp70-lacZ}
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General Information
Symbol
P{cutD-hsp70-lacZ}
FlyBase ID
FBtp0005532
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZct.D
Also Known As
ct-D2.3
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZct.D
Transgenic product class
Component
Use(s)
Regulatory region(s)
ct_cutD
Encoded product / tool
lacZ
Description

3.8kb HindIII fragment of the ct regulatory region is fused upstream of a minimal Hsp70 promoter followed by lacZ reporter sequence.

(Jack and DeLotto, 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
Malpighian tubule
(Jack and DeLotto, 1995)
ventral pharyngeal sense organ
(Jack and DeLotto, 1995)
larval antenno-maxillary sensory complex
(Jack and DeLotto, 1995)
presumptive embryonic/larval central nervous system | restricted
(Jack and DeLotto, 1995)
embryonic/larval labral segment sense organ
(Jack and DeLotto, 1995)
embryonic stage -- larval stage
fascicle
(Jack and DeLotto, 1995)
Additional Information
Statement
Reference

A 3.8kb HindIII fragment of the ctregulatory region fused to a minimal Hsp70 promoter drives expression ofEcollacZ in embryonic and larval tissues. In the embryonic central nervoussystem (CNS), EcollacZ expression is visible in 4 cells per segment. In theembryonic peripheral nervous system (PNS), EcollacZ is expressed in theantenno-maxillary organs, the hypophysis, and the epiphysis. The ventralend of fascicles which carry axons from the PNS into the ganglia of theCNS express EcollacZ in both embryos and late third instar larvae. A subsetof cells in the internal portion of the posterior spiracles of the embryoexpress EcollacZ. EcollacZ staining in the Malpighian tubules is weak.

(Jack and DeLotto, 1995)
 
Marker for
Reflects expression of
ct
(Jack and DeLotto, 1995)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{cutD-hsp70-lacZ}
(FlyBase, 1996-)
ct-D2.3
(Krattinger et al., 2007, Hu and Castelli-Gair, 1999)
Secondary FlyBase IDs
    References (6)