FlyBase Recombinant Construct Report: P{UAS-brm.K804R.T:HA1}

A 6.7kb fragment containing the brm coding sequence (with a K804R amino acid replacement in the ATP-binding site and which is tagged at the C-terminal end with Tag:HA), flanked by 66bp of 5' genomic DNA and approximately 1.3kb of 3' genomic DNA is expressed under the control of UASt regulatory sequences. The K804R change results in the expressed protein acting as a dominant-negative.

(Elfring et al., 1998)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

brm^{K804R.UAS.Tag:HA}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Brm-DN

(Li et al., 2016)

P[w⁺,UAS_GALhsp70:brm^K804R]

(Papoulas et al., 2001)

P[w⁺UAS_G-brm^K804R-HA-6His]

(Armstrong et al., 2002)

P{UAS-brm.K804R.T:HA1}

(FlyBase, 1996-)

UAS-Brm^DN

(Hong and Choi, 2016, Zhang et al., 2014, Kirilly et al., 2011)

UAS-Brm^K804R

(Bunker et al., 2015)

UAS-Mi-brm^DN

(Rives-Quinto et al., 2020)

UAS-brm-DN

(Roesley et al., 2018, Dietz et al., 2015)

UAS-brm^DN

(Bunker et al., 2015, Janssens et al., 2014, Herr et al., 2010)

UAS-brm^K804R

(Li et al., 2016, Morimoto et al., 2016, Dietz et al., 2015, Kwok et al., 2015, Herr et al., 2010, Corona et al., 2007)

UAS-brm[K804R]

(Armstrong et al., 2005, Krupp et al., 2005, Marenda et al., 2004, Moshkin et al., 2002, Mollaaghababa et al., 2001, Papoulas et al., 2001, Collins and Treisman, 2000, Collins et al., 1999)

UASBrm^K804R

(Zraly et al., 2006)

UASbrm^K804R

(Ivaldi et al., 2007)

Secondary FlyBase IDs

References (27)

Report Sections

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