The polyadenylation signal present in Tn\neoRRT between the Tn\neoR coding region and the promoter has been spliced out, so that the Tn\neoR gene is active.
report comment: Tn\neoRR lacks the polyA site present in Tn\neoRRT. Supports theory that I-element transposition occurs by reverse transcription via an RNA intermediate.
construct_comment: A heterogeneous class of elements, derived from transposition of P{I{neoRTW+}}. 3\'ends are conserved (with slight variation in number of TAA repeats), but 5\' truncations of several kilobases observed in 2 out of 3 cases.
report comment: Tn\neoRR lacks the polyA site present in Tn\neoRRT. Supports theory that I-element transposition occurs by reverse transcription via an RNA intermediate.
construct_comment: A heterogeneous class of elements, derived from transposition of P{I{neoRTW+}}. 3\'ends are conserved (with slight variation in number of TAA repeats), but 5\' truncations of several kilobases observed in 2 out of 3 cases.