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General Information
Symbol
P{I(neoRTW+)}
FlyBase ID
FBtp0005038
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Component allele Tn\neoRRT
Description

Construct: Contains the Tn\neoR gene downstream of an Hsp70 promoter in tandem with a Hsim\TK promoter. A polyadenylation signal is inserted between the promoter and the Tn\neoR coding region, preventing transcription of the Tn\neoR coding region. The polyadenylation signal is bracketed by a splice donor and splice acceptor site.

Component allele I-element\gagIR3
Description

Construct: A 6.2kb SalI fragment which includes the complete I-element.

Construct: Contains a full length I-element derived from wIR3, which has Tn\neoRRT inserted (without deletion of I-element sequences) 3' of the 2nd ORF of the transposase at 5214bp (coordinates according to FBrf0043891).

Component allele I-element\RTaseIR3
Description

Construct: A 6.2kb SalI fragment which includes the complete I-element.

Construct: Contains a full length I-element derived from wIR3, which has Tn\neoRRT inserted (without deletion of I-element sequences) 3' of the 2nd ORF of the transposase at 5214bp (coordinates according to FBrf0043891).

Component allele w+mW.hs
Description

Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.

Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Molecular data

Construct: Contains a full length I-element derived from wIR3, which has Tn\neoRRT inserted (without deletion of I-element sequences) 3' of the 2nd ORF of the transposase at 5214bp (coordinates according to FBrf0043891).

Construct: A 6.2kb SalI fragment which includes the complete I-element.

Molecular data

Construct: Contains a full length I-element derived from wIR3, which has Tn\neoRRT inserted (without deletion of I-element sequences) 3' of the 2nd ORF of the transposase at 5214bp (coordinates according to FBrf0043891).

Construct: A 6.2kb SalI fragment which includes the complete I-element.

Allele
Molecular data

Construct: Contains the Tn\neoR gene downstream of an Hsp70 promoter in tandem with a Hsim\TK promoter. A polyadenylation signal is inserted between the promoter and the Tn\neoR coding region, preventing transcription of the Tn\neoR coding region. The polyadenylation signal is bracketed by a splice donor and splice acceptor site.

Allele
Reference(s)
Molecular data

Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene.

Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

report comment: Tn\neoRRT not functional: contains polyA site located between the promoter and the coding neoR sequences.

construct_comment: Progenitors are those of the plasmid, pP{I{neoRTW+}}.

Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{I(neoRTW+)}
P{IneoRTW+}
P{I{neoRTW+}}
Secondary FlyBase IDs
    References (3)