The wgl-12 cDNA is separated from UASt regulatory sequences by an FRT cassette consisting of two FRT sites flanking an Hsp70 polyA site.
report comment: wg component of chimeric construct produces a temperature-sensitive protein.
report comment: Allele wgl-12.UAS is the FRT-deleted (active) form, carried on descendant transposon P{UAS-wg.ts}.
construct_comment: Contains Hsp70Bb polyA sequence between FRT sites, to prevent transcription of wgts in embryos (following injection).
report comment: wg component of chimeric construct produces a temperature-sensitive protein.
report comment: Allele wgl-12.UAS is the FRT-deleted (active) form, carried on descendant transposon P{UAS-wg.ts}.
construct_comment: Contains Hsp70Bb polyA sequence between FRT sites, to prevent transcription of wgts in embryos (following injection).