Construct: BamHI genomic fragment from coordinates +0.5 to -8 (0 is defined by an EcoRI site just upstream of the Cyp4d1 transcript) containing Cyp4d1 and the 5' half of wapl fused to cDNA sequences corresponding to the C-terminal sequences of the wapl transcript. The construct should encode the wapl 6.5kb cDNA expressed under the control of its normal promoter and upstream regulatory sequences.
BamHI genomic fragment from coordinates +0.5 to -8 (0 is defined by an EcoRI site just upstream of the Cyp4d1 transcript) containing Cyp4d1 and the 5' half of wapl fused to cDNA sequences corresponding to the C-terminal sequences of the wapl transcript. The construct should encode the wapl 6.5kb cDNA expressed under the control of its normal promoter and upstream regulatory sequences.