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General Information
Symbol
P{Ddc.E(spl)m8-HLH-lacZ.Gbe}
FlyBase ID
FBtp0014034
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
Gbe+Su(H)-lacZ, Su(H)GBE-lacZ, Su(H)-lacZ, NRE-lacZ, Gbe-Su(H)m8-lacZ, GBE-Su(H)-lacZ, Su(H)-GBE-lacZ, Gbe+Su(H)m8, Gbe+Su(H)lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Description

3 copies of the grh protein binding element Gbe (derived from the Ddc gene) and 2 Su(H) binding sites from the E(spl)m8-HLH gene are fused upstream of a minimal Hsp70Bb promoter driving lacZ reporter expression.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Molecular data

3 copies of the grh protein binding element Gbe (derived from the Ddc gene) and 2 Su(H) binding sites from the E(spl)m8-HLH gene are fused upstream of a minimal Hsp70Bb promoter driving lacZ reporter expression.

Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

A reporter is used to indicate N signalling. In late third instar wing discs, there is a complex expression pattern including a prominent stripe at the dorsal ventral (DV) wing disc border and a two-cell-wide stripe perpendicular to the DV boundary coincident with the anterior posterior (AP) organizer. The staining is on the ci-expressing side of the border indicating expression on the anterior side of the AP border. As development advances, expression decreases at the dorsal ventral border and increases at the AP border. When the pupal wing has fully everted, a number of stripes of lacZ expression parallel to the AP border are visible; the strongest of these stripes is at the AP border.

Ecol\lacZDdc.be2.E(spl)m8-HLH transcript is observed in peripheral cells, a cell in the larval midgut that extends processes around midgut imaginal islands.

The N signaling reporter is expressed at multiple steps in the follicle stem cell (FSC) lineage. It is expressed in an FSC and a cross-migrating FSC daughter, a cell between the two region 2b cysts, a cell at the posterior of the second region 2b cyst, multiple stage 6 follicle cells, polar cells, and cap cells.

Ecol\lacZDdc.be2.E(spl)m8-HLH protein is observed in 18% of small cells that occupy a basal position within the adult midgut epithelium.

Ecol\lacZDdc.be2.E(spl)m8-HLH protein is observed in half of Scer\GAL4NP5130-expressing cells in the adult midgut epithelium.

In third instar larva, high levels of expression in the imaginal discs, in pattern similar to E(spl)mβ; high levels at tracheal branchpoints.

 
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (35)
Reported As
Symbol Synonym
Gbe+Su(H)-lacZ
GBE+Su(H)-lacZ
Gbe+ Su(H)LacZ
Gbe+Su(H)m8+lacZ
Gbe+Su(H)m8-lacZ
Gbe+Su(H)m8
Gbe+Su(H)m8 reporter construct
Gbe+Su(H)m8-lacZ
Gbe-Su(H)-lacZ
Gbe+Su(H)LacZ
Gbe+Su(H)lacZ
P{Ddc.E(spl)-lacZ.Gbe.m8}
P{Ddc.E(spl)m8-HLH-lacZ.Gbe}
Su(H)-GBE::lacZ
Su(H)m8-lacZ
Su(H)GBE-LacZ
grh2,5-Su(H)x4lacZ
Secondary FlyBase IDs
    References (62)