Construct: NheI-EagI genomic fragment containing the entire tsu coding region. The fragment also contains most of the Mys45A coding region (but lacks the most 5' Mys45A sequences including the predicted start codon) and the 5' end of the Pmm45A gene (which is truncated just 3' of the predicted start codon).
Construct: NheI-EagI genomic fragment containing the entire tsu coding region. The fragment also contains most of the Mys45A coding region (but lacks the most 5' Mys45A sequences including the predicted start codon) and the 5' end of the Pmm45A gene (which is truncated just 3' of the predicted start codon).