GMR regulatory sequences drive expression of the complete rl coding sequence, followed by amino acids 2-147 of Scer\GAL4 (includes the DNA-binding domain), followed by residues 413-490 of Hsim\VP16 (activation domain), followed by a Tag:HSV-1-gD epitope tag. The fusion protein is tagged at the N-terminal end with a nuclear localisation signal (Tag:NLS(SV40-largeT)).