There are 11 nucleotide differences between Dmau\mariner\Tpch and a functional mariner transposase; 3 changes upstream of the open reading frame, four missense and one silent substitution within the open reading frame, and 3 changes downstream of the open reading frame.
construct_comment: Vector containing an almost complete duplication of M{peach} in head-to-head orientation. w+mW.hs is inserted at the junction of the duplicated fragments. The innermost pair of inverted repeats (directly adjacent to the w+mW.hs marker gene) are modified and are not susceptible to excision by Dmau\mariner\T, while the outermost inverted repeats are intact, and can, in principle, be used by Dmau\mariner\T to mobilise the vector.
Vector containing an almost complete duplication of M{peach} in head-to-head orientation. w+mW.hs is inserted at the junction of the duplicated fragments. The innermost pair of inverted repeats (directly adjacent to the w+mW.hs marker gene) are modified and are not susceptible to excision by Dmau\mariner\T, while the outermost inverted repeats are intact, and can, in principle, be used by Dmau\mariner\T to mobilise the vector.