FB2026_03 , released September 17, 2026
Recombinant Construct: P{Gr66a-GFP.IRES.GFP.IRES.GFP}
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General Information
Symbol
P{Gr66a-GFP.IRES.GFP.IRES.GFP}
FlyBase ID
FBtp0020702
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPGr66a.3x.IRES.2x
Also Known As
Gr66a-GFP, Gr66a-I-GFP, Gr66a-IRES-GFP, Gr66a-GFP-IRES-GFP-IRES-GFP
Size
Expression Data
GFP reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPGr66a.3x.IRES.2x
Transgenic product class
Component
Use(s)
Regulatory region(s)
Gr66a
Encoded product / tool
GFP
Description

A 3.15kb fragment including the Gr66a regulatory drives expression of three copies of GFP separated by IRES sites.

(Wang et al., 2004)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval gustatory receptor neuron of the dorsal pharyngeal sense organ | subset
(Mishra et al., 2013)
larval gustatory receptor neuron of the ventral pharyngeal sense organ | subset
(Mishra et al., 2013)
larval gnathal ganglion | restricted
(Mishra et al., 2013)
gustatory receptor neuron of terminal organ | subset
(Mishra et al., 2013)
high salt/bitter-sensing neuron | subset of terminal organ
(Alves et al., 2014)
gustatory receptor neuron of terminal organ dorsolateral group | subset
(Alves et al., 2014)
adult stage
high salt/bitter-sensing neuron of labellar taste bristle
(Miyamoto et al., 2012, Gordon and Scott, 2009)
gustatory receptor neuron of labellar taste bristle | subset
(Fischler et al., 2007)
gustatory receptor neuron | subset of adult gnathal ganglion
(Fischler et al., 2007)
high salt/bitter-sensing neuron
(Cameron et al., 2010)
gustatory receptor neuron | subset of labellar taste bristle
(Cameron et al., 2010)
gustatory receptor neuron of proboscis
(Wang et al., 2004)
Additional Information
Statement
Reference

AvicGFPGr66a.3x.IRES.2x is expressed in four gustatory receptor neurons of the terminal organ, which co-express ScerGAL4sano, a marker for salt-responsive neurons. Expression is also driven in two terminal organ neurons of the dorsal organ ganglion.

(Alves et al., 2014)

AvicGFPGr66a.3x.IRES.2x is expressed in gustatory receptor neurons of the dorsal and ventral the larval pharyngeal sense organ projecting to the embryonic/larval subesophageal ganglion.

(Mishra et al., 2013)

AvicGFPGr66a.3x.IRES.2x labels the bitter-sensing neurons (L2 GRN) of the taste bristles of the proboscis. Neurons labeled with AvicGFPGr66a.3x.IRES.2x do not overlap with expression of ScerGAL4ppk28.PC, which labels the water-sensing neurons.

(Cameron et al., 2010)

The dendrites of the AvicGFPGr66a.3x.IRES.2x bitter-sensitive neurons of gustatory sensilla and the ScerGAL4E49 labelled motor neurons are not in close proximity.

(Gordon and Scott, 2009)

AvicGFPGr66a.3x.IRES.2x is expressed in the bitter-sensitive gustatory receptor neurons of the labellar taste bristles of the proboscis. Expression of AvicGFPGr66a.3x.IRES.2x in the proboscis is distinct from the expression of ScerGAL4E409. The projection of the neurons labeled by AvicGFPGr66a.3x.IRES.2x in the subesophageal ganglion does not overlap with the projections labeled by ScerGAL4E409.

(Fischler et al., 2007)
 
Marker for
high salt/bitter-sensing neuron
(Jeong et al., 2013, Miyamoto et al., 2012, Cameron et al., 2010)
Reflects expression of
Gr66a
(Alves et al., 2014, Miyamoto et al., 2012, Cameron et al., 2010, Gordon and Scott, 2009, Fischler et al., 2007, Wang et al., 2004)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (20)