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General Information
Symbol
P{Gr66a-GFP.IRES.GFP.IRES.GFP}
FlyBase ID
FBtp0020702
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
Gr66a-GFP, Gr66a-I-GFP, Gr66a-IRES-GFP, Gr66a-GFP-IRES-GFP-IRES-GFP
Size
Expression Data
GFP reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Avic\GFPGr66a.3x.IRES.2x
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Description

A 3.15kb fragment including the Gr66a regulatory drives expression of three copies of GFP separated by IRES sites.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
Component Alleles
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Avic\GFPGr66a.3x.IRES.2x is expressed in four gustatory receptor neurons of the terminal organ, which co-express Scer\GAL4sano, a marker for salt-responsive neurons. Expression is also driven in two terminal organ neurons of the dorsal organ ganglion.

Avic\GFPGr66a.3x.IRES.2x is expressed in gustatory receptor neurons of the dorsal and ventral the larval pharyngeal sense organ projecting to the embryonic/larval subesophageal ganglion.

Avic\GFPGr66a.3x.IRES.2x labels the bitter-sensing neurons (L2 GRN) of the taste bristles of the proboscis. Neurons labeled with Avic\GFPGr66a.3x.IRES.2x do not overlap with expression of Scer\GAL4ppk28.PC, which labels the water-sensing neurons.

The dendrites of the Avic\GFPGr66a.3x.IRES.2x bitter-sensitive neurons of gustatory sensilla and the Scer\GAL4E49 labelled motor neurons are not in close proximity.

Avic\GFPGr66a.3x.IRES.2x is expressed in the bitter-sensitive gustatory receptor neurons of the labellar taste bristles of the proboscis. Expression of Avic\GFPGr66a.3x.IRES.2x in the proboscis is distinct from the expression of Scer\GAL4E409. The projection of the neurons labeled by Avic\GFPGr66a.3x.IRES.2x in the subesophageal ganglion does not overlap with the projections labeled by Scer\GAL4E409.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (18)