FB2020_06, released Dec 22, 2020

Recombinant Construct: P{Gr5a-GFP.IRES.GFP.IRES.GFP}

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General Information
Symbol
P{Gr5a-GFP.IRES.GFP.IRES.GFP}
FlyBase ID
FBtp0039241
Feature type
transgenic_transposon
Component Allele(s)
Avic\GFPGr5a.3x.IRES.2x
Also Known As
Gr5a-GFP-IRES-GFP-IRES-GFP, Gr5a-I-GFP
Size
Expression Data
GFP reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Fischler et al., 2007)
Construct components
Component allele Avic\GFPGr5a.3x.IRES.2x
Product class / Tool use(s)
Regulatory region(s)
Gr5a
Encoded product / tool
GFP
Description

Gr5a regulatory sequences drive expression of 3 copies of GFP separated by IRES sequences.

(Fischler et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
(Fischler et al., 2007)
Component Alleles
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
gustatory receptor neuron of labellar taste peg | subset
(Fischler et al., 2007)
gustatory receptor neuron | subset of adult gnathal ganglion
(Fischler et al., 2007)
S cell
(Cameron et al., 2010)
gustatory receptor neuron | subset of labellar taste bristle
(Cameron et al., 2010)
gustatory receptor neuron of the labellum | subset of posterior maxillary sensory center
(Miyazaki and Ito, 2010)
Additional Information
Statement
Reference

Avic\GFPGr5a.3x.IRES.2x labels the sugar-sensing neurons (S cell) of the taste bristles of the proboscis. Neurons labeled with Avic\GFPGr5a.3x.IRES.2x do not overlap with expression of Scer\GAL4ppk28.PC, which labels the water-sensing neurons.

(Cameron et al., 2010)

Avic\GFPGr5a.3x.IRES.2x is expressed in a subset of sugar-responsive gustatory receptor neurons of the labellum that project to regions PMS4 and PMS5 of the posterior maxillary sensory center. The projections pattern overlaps with the one of Scer\GAL4NP1017.

(Miyazaki and Ito, 2010)

Avic\GFPGr5a.3x.IRES.2x is expressed in the sugar-sensitive gustatory receptor neurons of the labellar taste pegs of the proboscis. Expression of Avic\GFPGr5a.3x.IRES.2x in the proboscis is distinct from the expression of Scer\GAL4E409. The projection of the neurons labeled by Avic\GFPGr5a.3x.IRES.2x in the anterior subesophageal ganglion does not overlap with the projections labeled by Scer\GAL4E409.

(Fischler et al., 2007)
 
Marker for
S cell
(Cameron et al., 2010)
Reflects expression of
Gr5a
(Cameron et al., 2010, Miyazaki and Ito, 2010, Fischler et al., 2007)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Gr5a-GFP-IRES-GFP-IRES-GFP
(Cameron et al., 2010, Miyazaki and Ito, 2010, Fischler et al., 2007)
Gr5a-I-GFP
(Leung et al., 2020, Zhang et al., 2013)
Gr5a-gfp-IRES-gfp-IRES-gfp
(Flood et al., 2013)
P{Gr5a-GFP.IRES.GFP.IRES.GFP}
(FlyBase, 1996-)
P{Tre-GFP.IRES.GFP.IRES.GFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (9)