FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{Gr5a-GFP.IRES.GFP.IRES.GFP}
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General Information
Symbol
P{Gr5a-GFP.IRES.GFP.IRES.GFP}
FlyBase ID
FBtp0039241
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPGr5a.3x.IRES.2x
Also Known As
Gr5a-GFP-IRES-GFP-IRES-GFP, Gr5a-I-GFP
Size
Expression Data
GFP reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPGr5a.3x.IRES.2x
Transgenic product class
Component
Use(s)
Regulatory region(s)
Gr5a
Encoded product / tool
GFP
Description

Gr5a regulatory sequences drive expression of 3 copies of GFP separated by IRES sequences.

(Fischler et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
gustatory receptor neuron of labellar taste peg | subset
(Fischler et al., 2007)
gustatory receptor neuron | subset of adult gnathal ganglion
(Fischler et al., 2007)
sugar-sensing neuron of taste bristle
(Cameron et al., 2010)
gustatory receptor neuron | subset of labellar taste bristle
(Cameron et al., 2010)
gustatory receptor neuron of the labellum | subset of posterior maxillary sensory center
(Miyazaki and Ito, 2010)
Additional Information
Statement
Reference

AvicGFPGr5a.3x.IRES.2x labels the sugar-sensing neurons (S cell) of the taste bristles of the proboscis. Neurons labeled with AvicGFPGr5a.3x.IRES.2x do not overlap with expression of ScerGAL4ppk28.PC, which labels the water-sensing neurons.

(Cameron et al., 2010)

AvicGFPGr5a.3x.IRES.2x is expressed in a subset of sugar-responsive gustatory receptor neurons of the labellum that project to regions PMS4 and PMS5 of the posterior maxillary sensory center. The projections pattern overlaps with the one of ScerGAL4NP1017.

(Miyazaki and Ito, 2010)

AvicGFPGr5a.3x.IRES.2x is expressed in the sugar-sensitive gustatory receptor neurons of the labellar taste pegs of the proboscis. Expression of AvicGFPGr5a.3x.IRES.2x in the proboscis is distinct from the expression of ScerGAL4E409. The projection of the neurons labeled by AvicGFPGr5a.3x.IRES.2x in the anterior subesophageal ganglion does not overlap with the projections labeled by ScerGAL4E409.

(Fischler et al., 2007)
 
Marker for
sugar-sensing neuron of taste bristle
(Cameron et al., 2010)
Reflects expression of
Gr5a
(Cameron et al., 2010, Miyazaki and Ito, 2010, Fischler et al., 2007)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Gr5a-GFP-IRES-GFP-IRES-GFP
(Cameron et al., 2010, Miyazaki and Ito, 2010, Fischler et al., 2007)
Gr5a-I-GFP
(Leung et al., 2020, Zhang et al., 2013)
Gr5a-gfp-IRES-gfp-IRES-gfp
(Flood et al., 2013)
P{Gr5a-GFP.IRES.GFP.IRES.GFP}
(FlyBase, 1996-)
P{Tre-GFP.IRES.GFP.IRES.GFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (10)