FB2026_03 , released September 17, 2026
Recombinant Construct: P{FRT(Dpy-30L2.YFP)}
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General Information
Symbol
P{FRT(Dpy-30L2.YFP)}
FlyBase ID
FBtp0041048
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPYFP.Dpy-30L2.Scer\SceI.RS
Size
Expression Data
GFP reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

The P{FRT(Dpy-30L2.YFP)} construct is a targeting construct for disruption of the Dpy-30L2 gene by homologous recombination and can also be used to study the expression of YFP under the influence of the Dpy-30L2 regulatory sequences in the construct.

(Vardanyan et al., 2008)
Construct components
Component
Use(s)
Also carries
RS(I-SceI)
Component allele Avic\GFPYFP.Dpy-30L2.Scer\SceI.RS
Transgenic product class
Component
Use(s)
Regulatory region(s)
Dpy-30L2
Encoded product / tool
YFP
Description

Contains a DNA segment from the Dpy-30L2 locus, with 3.1kb of upstream and 3.6kb of downstream sequences. The coding sequence of YFP with its genuine stop codon followed by the SV40 polyadenylation/transcription termination sequence has been inserted in frame immediately following the ATG start codon of Dpy-30L2. The insertion of YFP results in a disruption of the Dpy-30L2 reading frame as well as a deletion of 40 amino acids from the coding region.

(Vardanyan et al., 2008)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
gonad
(Vardanyan et al., 2008)
adult stage | male
mid-late elongation-stage spermatid
(Vardanyan et al., 2008)
Additional Information
Statement
Reference

Expressed in elongated spermatids at the "canoe-like" stage but not during the early stages of spermatogenesis or in mature sperm.

(Vardanyan et al., 2008)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{FRT(Dpy-30L2.YFP)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)