FB2026_02 , released June 18, 2026
Recombinant Construct: P{Prosα3-EGFP.M}
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General Information
Symbol
P{Prosα3-EGFP.M}
FlyBase ID
FBtp0050454
Feature type
transgenic_transposable_element
Component Allele(s)
Prosα3EGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele Prosα3EGFP
Transgenic product class
wild_type
(Ma et al., 2002)
Component
Use(s)
Regulatory region(s)
Prosα3
Encoded product / tool
Prosα3
Tagged with
EGFP
Description

Contains the Prosα3 gene, with 2.2kb of upstream sequences and 2.2kb of downstream sequences. The Prosα3 open reading frame is tagged at the C-terminal end with EGFP.

(Ma et al., 2002)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage -- third instar larval stage
organism | ubiquitous
(Ma et al., 2002)
imaginal disc | ubiquitous
(Ma et al., 2002)
larval central nervous system | ubiquitous
(Ma et al., 2002)
embryonic/larval salivary gland | ubiquitous
(Ma et al., 2002)
ovary | ubiquitous
(Ma et al., 2002)
testis | ubiquitous
(Ma et al., 2002)
spermatogenesis
spermatogonium
(Ma et al., 2002)
primary spermatocyte
(Ma et al., 2002)
secondary spermatocyte
(Ma et al., 2002)
spermatid
(Ma et al., 2002)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
mid-late elongation-stage spermatid
(Rathke et al., 2007)
Additional Information
Statement
Reference

Observed in early and late canoe-stage spermatids and vanishes in late canoe stage.

(Rathke et al., 2007)

Zygotic expression of Pros29T:AvicGFP-EGFP is first observed at the second larval instar, and is detected in all tissues. Pros29T:AvicGFP-EGFP expression is observed at all stages of spermatogenesis.

(Ma et al., 2002)
 
Marker for
Reflects expression of
Prosα3
(Rathke et al., 2007, Ma et al., 2002)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Prosα3-GFP
(Zhong and Belote, 2007)
Prosα3-eGFP
(Rathke et al., 2007)
P{Prosα3-EGFP.M}
(FlyBase, 1992-)
pW8/Prosα3-GFP
(Ma et al., 2002)
Secondary FlyBase IDs
    References (5)