FlyBase Recombinant Construct Report: P{fwe-8.2g-FLAG-HA-MYC}

An 8.2kb fragment covering the fwe gene locus, modified such that Tag:FLAG is inserted downstream of the -RA transcript, a Tag:HA epitope is tagged onto the C-terminal of -RB and a Tag:MYC epitope is inserted downstream of the -RC isoform.

(Yao et al., 2009)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

fwe^{+8.2.RA.Tag:FLAG.RB.Tag:HA.RC.Tag:MYC}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

neuropil of presumptive embryonic/larval central nervous system

(Yao et al., 2009)

third instar larval stage

neuropil of larval brain

(Yao et al., 2009)

adult stage

neuropil of adult brain

(Yao et al., 2009)

Additional Information

Statement

Reference

Only the epitope-tagged fwe-PB isoform is detectable in the neuropil of embryonic, larval and adult central nervous system.

(Yao et al., 2009)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

51616

w^*; P{fwe-8.2g-FLAG-HA-MYC}3-3

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P{ w⁺ gen res [Flag/HA/Myc]}

(Yao et al., 2009)

P{flower-8.2g-FLAG-HA-MYC}

(FlyBase, 1992-)

P{fwe-8.2g-FLAG-HA-MYC}

(FlyBase, 1996-)

flower^LoseA-HA

(Coelho et al., 2018)

flower^LoseB-myc

(Coelho et al., 2018)

flower^ubi-flag

(Coelho et al., 2018)

gen res[Flag/HA/Myc]

(Yao et al., 2009)

Secondary FlyBase IDs

References (6)

Report Sections

Open Close