The FRT cassette present in P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K4A.HA} has been excised (removing wild-type His3.3B sequences present within the cassette), allowing the mutated form of His3.3B (which contains the amino acid replacement K4A and which is also tagged at the C-terminal end with Tag:HA) to be expressed under the control of the His3.3B promoter.
The Scer\FRT cassette present in P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K4A.HA} has been excised (removing wild-type His3.3B sequences present within the cassette), allowing the mutated form of His3.3B (which contains the amino acid replacement K4A and which is also tagged at the C-terminal end with T:Ivir\HA1) to be expressed under the control of the His3.3B promoter (this mutated form of His3.3B is represented by His3.3BK4A.T:Ivir\HA1).