In the larval CNS, ScerGAL4^Lk.2.2 drives expression in five pairs of neurons in the brain, including four pairs of ipc-1 neurons that display ITP instead of Lk immunoreactivity; three pairs of neurons in the subesophageal ganglion, and seven pairs of neurons in the ventral nerve cord. In the adult CNS, ScerGAL4^Lk.2.2 drives expression in one pair of neurons in the lateral horn, one pair of neurons in the gnathal ganglion (adult SOG), four pairs of brain neurons that display ITP immunoreactivity, and eleven pairs of neurons in the thoracico-abdominal ganglion. Expression in the adult ipc-1 neurons is weak and variable. All the larval ABLK neurons co-express Dh44 protein and the driver ScerGAL4^GMR65C11; four to eight anterior adult ABLK neurons also co-express Dh44 protein and the driver ScerGAL4^GMR65C11.

ScerGAL4^Lk.2.2 drives expression in adult Leucokinin LHKL lateral horn neurons. Dendrites, defined by a dendritic marker, of these neurons intermingle with projections from s-LNv neurons, while synapses, defined by a presynaptic marker, are in more posterior parts of the neuron that do not intermingle with s-LNv neurons.

ScerGAL4^Lk.2.2 is expressed in three to four neurons in the lateral protocerebrum of larval brains, named as the ALK neurons. ScerGAL4^Lk.2.2 is expressed in a second group of neurons called the LHLK cells located on the lateral horn in adults and an equivalent position in larvae. ScerGAL4^Lk.2.2 is expressed in the SELK neurons, which are present in group sof two or three pairs in the subesophageal ganglion. Seven pairs of abdominal neurons, called the LK cells, express ScerGAL4^Lk.2.2.