In the absence of a GAL4 driver, the P{UAS-DTH2} construct shows significant rescue of flies that lack ple function in the central nervous system.
UASt regulatory sequences are fused upstream of a fragment that encodes the entire ple transcription unit, where all exons are present but all intronic regions have been removed. This results in a transgene which expresses only the type 2 (non-neural) isoform.