FB2025_05 , released December 11, 2025
Recombinant Construct: P{UAS-eGFP.mir-8.sponge}
Open Close
General Information
Symbol
P{UAS-eGFP.mir-8.sponge}
FlyBase ID
FBtp0070469
Feature type
transgenic_transposable_element
Component Allele(s)
mir-8sponge.UAS.EGFP
Also Known As
UAS-mir-8-Sponge(SP)::GFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele mir-8sponge.UAS.EGFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-8
Tagged with
EGFP
Description

UAS regulatory sequences drive expression of a mir-8 "sponge" sequence, which consists of ten repetitive mir-8 complementary sequences with mismatches at positions 9-12 separated by a variable four-nucleotide linker. The sponge sequence is inserted into 3'UTR sequence downstream of the EGFP coding region, to enhance stability.

(Loya et al., 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (15)
Reported As
Symbol Synonym
P{UAS-eGFP.mir-8.sponge}
(FlyBase, 1996-)
P{UAS-eGFP.mir-8SP}
(FlyBase, 1992-)
UAS-GFPmiR-8SP#10
(Kennell et al., 2012)
UAS-GFPmiR-8SP#9
(Kennell et al., 2012)
UAS-GFPmiRNA
(Lin et al., 2013)
UAS-miR-8 SP
(Nesler et al., 2013)
UAS-miR-8 sponge
(Loya et al., 2009)
UAS-miR-8-sponge-GFP
(Sander et al., 2018)
UAS-miR-8SP
(Kennell et al., 2012)
UAS-mir-8-SP::GFP
(Morante et al., 2013)
UAS-mir-8-Sponge(SP)::GFP
(Morante et al., 2013)
UAS-mir-8-sponge
(Antonello et al., 2015)
UAS-mir-8-sponge(SP)::GFP
(Antonello et al., 2015)
miR-8SP
(Loya et al., 2009)
miR8sp10
(Loya et al., 2009)
Secondary FlyBase IDs
    References (10)