ScerGAL4^Ast.2.1 drives expression in a group of neurons in the adult CNS. Per hemisphere, it labels 6 neurons in the central brain which innervate the the subesophageal ganglion, pars intercerebralis and protocerebrum. Around 30 optic lobe neurons are labeled, innervating the medulla. Three neurons in the thoracico-abdominal ganglion are labeled by ScerGAL4^Ast.2.1; their dorsal projections innervate the lower midgut, the hindgut and the rectum. ScerGAL4^Ast.2.1 also drives expression in cells in the upper midgut and midgut transitions and 2 peripheral neurons per hemisegment.