FlyBase Allele Report: Scer\GAL4[AstA.2.1]

General Information

Symbol

Scer\GAL4^AstA.2.1

Species

S. cerevisiae

Name

FlyBase ID

FBal0267978

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{AstA-GAL4.2.1}

Also Known As

AstA-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Hergarden et al., 2012)

Progenitor genotype

Carried in construct

P{AstA-GAL4.2.1}

Cytology

Description

A 2096bp fragment from upstream of the putative AstA transcriptional start site is fused upstream of sequence encoding a GAL4 driver.

(Hergarden et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

AstA

AstA_2096bp

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

ScerGAL4^Ast.2.1 drives expression in a group of neurons in the adult CNS. Per hemisphere, it labels 6 neurons in the central brain which innervate the the subesophageal ganglion, pars intercerebralis and protocerebrum. Around 30 optic lobe neurons are labeled, innervating the medulla. Three neurons in the thoracico-abdominal ganglion are labeled by ScerGAL4^Ast.2.1; their dorsal projections innervate the lower midgut, the hindgut and the rectum. ScerGAL4^Ast.2.1 also drives expression in cells in the upper midgut and midgut transitions and 2 peripheral neurons per hemisegment.

(Hergarden et al., 2012)

Marker for

Reflects expression of

AstA

(Hergarden et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Acute thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^AstA.2.1 cells, in combination with a paired odor, in adults results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

Inhibition (using shi^ts.UAS.cUa at 33[o]C) of Scer\GAL4^AstA.2.1 cells in adults significantly reduces short-term associative appetitive olfactory memory, compared to controls, with no effect on sugar preference.

(Yamagata et al., 2016)

Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine} with orange light) of Scer\GAL4^AstA.2.1 cells, significantly reduces food intake of satiated flies, compared to controls; activation the combined set of Scer\GAL4^Gr28b.b.4.7 and Scer\GAL4^AstA.2.1 cells in adults significantly increases food intake of satiated flies, compared to controls, but this is to a significantly reduced extent compared to Scer\GAL4^Gr28b.b.4.7 cells alone.

(Zhan et al., 2016)

Increasing neuronal excitability of Scer\GAL4^AstA.2.1 neurons leads to flies showing a preference for a protein rich yeast diet, compared to controls (this is more pronounced in females than males).

(Hentze et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Bhal\NaChBac^UAS.cNa, Scer\GAL4^AstA.2.1 has abnormal starvation stress response phenotype, suppressible by Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^AstA.2.1

(Hergarden et al., 2012)

Suppressor of

Statement

Reference

Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^AstA.2.1 is a suppressor of abnormal starvation stress response phenotype of Bhal\NaChBac^UAS.cNa, Scer\GAL4^AstA.2.1

(Hergarden et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51979

w¹¹¹⁸; P{AstA-GAL4.2.1}5

51978

w¹¹¹⁸; P{AstA-GAL4.2.1}3M/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Ast.2.1

Scer\GAL4^AstA.2.1

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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