FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-Oamb-Tango}
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General Information
Symbol
P{UAS-Oamb-Tango}
FlyBase ID
FBtp0072867
Feature type
transgenic_transposable_element
Component Allele(s)
Arr1::TEV\NIaUAS.Tango
OambUAS.Tango
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Arr1::TEV\NIaUAS.Tango
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
TEVp
Description

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of either the Dop1R1 or Oamb receptor (depending on the particular construct) fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the Dop1R1UAS.Tango and OambUAS.Tango alleles respectively). The downstream coding region consists of Arr1 fused to the TEVp protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Dop1R1 or Oamb receptor by its ligand, the Arr1 ::TEVp protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

(Inagaki et al., 2012)
Component allele OambUAS.Tango
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Oamb
Tagged with
lexA::VP16
Tag:CS(TEVp)
Tag:HA
Description

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of the Oamb receptor fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the OambUAS.Tango allele). The downstream coding region consists of Arr1 fused to the TEVp protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Oamb receptor by its ligand, the Arr1 ::TEVp protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

(Inagaki et al., 2012)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

The P{UAS-Oamb-Tango} construct can be used as a reporter of octopamine release in vivo.

(Inagaki et al., 2012)
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
68235
y1 v1; P{UAS-Oamb-Tango}attP2/TM3, Ser1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
OctR-Tango
(Inagaki et al., 2012)
P{UAS-Oamb-Tango}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)