A 1788bp fragment comprising the intergenic region between CG14402 and twit is fused upstream of sequence encoding a GAL4 driver. The first intron of twit (3328bp) is present downstream of the GAL4 coding region.
ScerGAL4twit.UIR.INT drives expression in the motorneurons in the ventral nerve cord, where it colocalises with phosphorylated Mad protein.