A 3214 base pair fragment from the flanking non-coding or intronic region of beat-IIa is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
A FLP/FRT intersectional strategy shows expression of EcollexAGMR48F07 in hg1 motor neuron when combined with ScerGAL4GMR73C03.