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General Information
Symbol
P{lexAop-UAS-mKate2.CAAX.K}
FlyBase ID
FBtp0093003
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Description

The P{lexAop-UAS-mKate2.CAAX.K} construct was generated in vivo, by activation of the P{Raeppli-CAAX} construct, a two-step process in which FLP recombinase is first used to allow expression of the phiC31:int coding region present in the starting construct, which then subsequently promotes site-specific recombination between a single attB site and one of several attP sites in the starting construct. This results in the stable selection of one of several fluorescent markers present in the starting construct (only the fluorescent protein brought closest to the regulatory sequences is expressed as a stop codon is present after each fluorescent marker coding region). The P{lexAop-UAS-mKate2.CAAX.K} construct expresses the mKate2 fluorescent protein.

Product class / Tool use(s)
Encoded product / tool
Description

lexAop and UAS regulatory sequences are followed by a minimal heat shock promoter and the mKate2 fluorescent protein tagged with a membrane targeting sequence (Tag:CAAX(Unk)). The lexAop and UAS regulatory sequences are flanked by loxP and lox2272 sites respectively (meaning that the P1cre recombinase can be used to excise one of the enhancers in a mutually exclusive manner if desired).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Molecular data

lexAop and UAS regulatory sequences are followed by a minimal heat shock promoter and the mKate2 fluorescent protein tagged with a membrane targeting sequence (Tag:CAAX(Unk)). The lexAop and UAS regulatory sequences are flanked by loxP and lox2272 sites respectively (meaning that the P1cre recombinase can be used to excise one of the enhancers in a mutually exclusive manner if desired).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{lexAop-UAS-mKate2.CAAX.K}
Secondary FlyBase IDs
    References (2)