FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-DD-GAL4.VP16.I}
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General Information
Symbol
P{UAS-DD-GAL4.VP16.I}
FlyBase ID
FBtp0093574
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4VP16.UAS.cIa
Transgenic product class
Component
Use(s)
Description

UAS regulatory sequences are fused upstream of sequence encoding the FKBP12 destabilization domain (Tag:degron(FKBP)) followed by GAL4::VP16 driver sequence. This construct was intended to result in a GAL4::VP16 driver whose degradation could be controlled chemically by the 'Shield1' small molecule (which can bind the Tag:degron(FKBP) sequence to prevent degradation, see PMID:16959577), but the destabilization domain does not appear to be effective in the P{UAS-DD-GAL4.VP16.I}CG60511 insertion of this construct. The P{UAS-DD-GAL4.VP16.I}CG60511 insertion also does not behave as expected with respect to UAS-driven expression, and it is not known whether other insertions of this construct will show the expected UAS-inducible expression.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

ScerGAL4VP16.UAS.cIa expresses GAL4 in the late pupal wing blade, and in larval muscles and trachea (presumably reflecting nearby enhancers), but no apparent UAS activity. Destabilization domain appears ineffective.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{UAS-DD-GAL4.VP16.I}
Secondary FlyBase IDs
    References (3)