The FRT cassette containing the GAL4 driver coding sequence has been excised from P{alrm(FRT.GAL4)lexA::GAD}, generating P{alrm(-FRT)lexA::GAD}, which expresses a lexA::GAD driver under the control of the alrm promoter sequences.
The Scer\FRT cassette containing the Scer\GAL4 coding region has been excised from P{alrm(FRT.GAL4)lexA::GAD}, generating P{alrm(-FRT)lexA::GAD}, which expresses a fusion protein consisting of Ecol\lexA and the Scer\GAL4 activation domain under the control of the alrm promoter sequences.
The Scer\FRT cassette containing the Scer\GAL4 coding region has been excised from P{alrm(FRT.GAL4)lexA::GAD}, generating P{alrm(-FRT)lexA::GAD}, which expresses a fusion protein consisting of Ecol\lexA and the Scer\GAL4 activation domain under the control of the alrm promoter sequences.