FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{R14C08-GAL4.DBD}
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General Information
Symbol
P{R14C08-GAL4.DBD}
FlyBase ID
FBtp0099458
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4DBD.R14C08
Also Known As
R14C08
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4DBD.R14C08
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR14C08
Encoded product / tool
GAL4(DBD)::Zip-
Description

The R14C08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
first instar larval stage -- third instar larval stage
larval mushroom body output neuron i1

Comment: when combined with P{R20C05-p65.AD} (combination referred to as 'SS01726')

(Eschbach et al., 2021)
third instar larval stage
larval mushroom body output neuron i1

Comment: when combined with P{R15B01-p65.AD} (combination referred to as 'MB141B')

when combined with P{R20C05-p65.AD} (combination referred to as 'SS01726')

when combined with P{R65A05-p65.AD} (combination referred to as 'SS01771')

(Truman et al., 2023, Eschbach et al., 2020, Saumweber et al., 2018)
adult stage
mushroom body output neuron 1

Comment: when combined with P{R12C11-p65.AD} (combination referred to as 'MB002B')

(Scaplen et al., 2021, Lewis et al., 2015, Aso et al., 2014)
mushroom body output neuron 3

Comment: when combined with P{R12C11-p65.AD} (combination referred to as 'MB002B')

(Scaplen et al., 2021, Dolan et al., 2018, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 1 | faint

Comment: when combined with P{R12C11-p65.AD} (combination referred to as 'MB002B')

(Aso et al., 2014)
lobula columnar neuron LC11

Comment: when combined with P{R22H02-p65.AD} (combination referred to as 'OL0016B')

(Klapoetke et al., 2022)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

(Lewis et al., 2015)
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{R14C08-GAL4.DBD}
(FlyBase, 1992-)
R14C08
(Truman et al., 2023, Eschbach et al., 2020, Jovanic et al., 2019, Saumweber et al., 2018, Aso et al., 2014)
R14C08-ZpGAL4DBD
(Klapoetke et al., 2022)
Secondary FlyBase IDs
    References (27)