FlyBase Recombinant Construct Report: P{Ubi-scra.RBD-mEGFP(A206K)}

General Information

Symbol

P{Ubi-scra.RBD-mEGFP(A206K)}

FlyBase ID

FBtp0111810

Feature type

transgenic_transposable_element

Component Allele(s)

scra^{RBD.Ubi-p63E.mEGFP(A206K)}

Also Known As

Ubi-GFP::AniRBD, Ubi-AniRBD::GFP, Rho1 sensor, Anillin-RBD-GFP, AniRBD-GFP, Ubi-Anillin-RBD-EGFP, ubi-aniRBD-GFP

Size

Expression Data

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele scra^{RBD.Ubi-p63E.mEGFP(A206K)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

Ubi-p63E

Encoded product / tool

scra

Tagged with

mEGFP(A206K)

green fluorescent protein

Description

The Ubi-p63E promoter drives expression of the Rho1 GTP-binding domain of scra (amino acids 748-1239 are present), tagged at the C-terminal end with a GSSGSS linker followed by mEGFP(A206K). This protein acts as a sensor for the activity of Rho1.

(Munjal et al., 2015)

Does not act as a Rho1- or Rho-family GTPase-specific biosensor; the encoded protein binds to both GDP- and GTP-bound Rho1, Cdc42, and, to a lesser extent, Rac1.

(Stjepić et al., 2024)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

scra^{RBD.Ubi-p63E.mEGFP(A206K)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

ANI-RBD::mEGFP

(Karki et al., 2023)

Ani-RBD::GFP

(Garcia De Las Bayonas et al., 2019)

AniRBD-GFP

(Deneke et al., 2019, Munjal et al., 2015)

AniRBD::GFP

(Kerridge et al., 2016)

AniRDB-GFP

(Doerflinger et al., 2022)

Anillin-RBD-GFP

(Mitra et al., 2026, Jiang and Harris, 2019)

Anillin:RBD:GFP

(di Pietro et al., 2023)

AnillinRBD-GFP

(Sharma and Rikhy, 2021)

P{Ubi-GFP.AniRBD}

(FlyBase, 1992-)

P{Ubi-scra.RBD-mEGFP(A206K)}

(FlyBase, 1996-)

RBD-GFP

(Greig and Bulgakova, 2020)

Rho1 sensor

(Schmidt et al., 2021, Segal et al., 2018)

Rho1GTP sensor

(Kerridge et al., 2016)

UAS-Anillin-RBD::GFP

(Silver et al., 2019)

Ubi-AniRBD-GFP

(Stjepić et al., 2024)

Ubi-AniRBD::GFP

(Segal et al., 2018, Munjal et al., 2015)

Ubi-Anillin-RBD-EGFP

(Schmidt et al., 2021)

Ubi-Anillin-RBD-eGFP

(Lehne and Bogdan, 2023)

Ubi-EGFP-AnillinRBD

(Sidor et al., 2020)

Ubi-GFP::AniRBD

(Garcia De Las Bayonas et al., 2019, Jha et al., 2018, Kerridge et al., 2016)

ubi-GFP:anillinRBD

(Rothenberg et al., 2023)

ubi-aniRBD-GFP

(Villars et al., 2022)

ubi-anilinRBD::GFP

(Kerridge et al., 2016)

ubi-anillin(RBD)::EGFP

(Bailles et al., 2019)

ubi-scra^RBD-GFP

(Toret et al., 2018)

ubi::AniRBD-GFP

(Greig and Bulgakova, 2020)

Secondary FlyBase IDs

References (25)

Report Sections

Open Close