FlyBase Recombinant Construct Report: PBac{Clk-V5-GFP}

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Recombinant Construct: PBac{Clk-V5-GFP}

General Information

Symbol

PBac{Clk-V5-GFP}

FlyBase ID

FBtp0112012

Feature type

transgenic_transposable_element

Component Allele(s)

Clk^Tag:V5,EGFP

Also Known As

Clk-GFP

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Clk^Tag:V5,EGFP

Transgenic product class

(Liu et al., 2015)

(Liu et al., 2015)

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Tagged with

green fluorescent protein

Description

An approximately 15kb genomic fragment containing Clk. Tag:V5 and EGFP tags have been introduced at the C-terminal end of the Clk coding region.

(Liu et al., 2015)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Clk^Tag:V5,EGFP

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

larval DN1 neuron

Comment: 48h AEL

(Liu et al., 2015)

larval DN2 neuron

Comment: 48h AEL

(Liu et al., 2015)

larval s-LNv Pdf neuron

Comment: 48h AEL

(Liu et al., 2015)

second instar larval stage

larval DN1 neuron

Comment: 60h AEL

(Liu et al., 2015)

larval DN2 neuron

Comment: 60h AEL

(Liu et al., 2015)

larval LNd neuron

Comment: 72h AEL

(Liu et al., 2015)

larval s-LNv Pdf neuron

Comment: 60h AEL

(Liu et al., 2015)

third instar larval stage

adult LP neuron

(Liu et al., 2015)

larval s-LNv Pdf neuron

(Liu et al., 2015)

larval LNd neuron

(Liu et al., 2015)

larval DN period neuron

(Liu et al., 2015)

pupal stage P6 -- P7

adult LP neuron

(Liu et al., 2015)

adult s-LNv neuron

(Liu et al., 2015)

pupal DN period neuron

(Liu et al., 2015)

(Liu et al., 2015)

pharate adult stage P8 -- adult stage

adult LP neuron

(Liu et al., 2015)

(Liu et al., 2015)

adult s-LNv neuron

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

Additional Information

Statement

Reference

In all stages expression was analyzed at zeitgeber time 22 as well as at zeitgeber time 12 in the third instar larval stage. Clk^{T:SV5V5,T:AvicGFP-EGFP} is detected in around eight neurons per hemisphere in L1 and L2 larvae. This number increases to around 60 cells per hemisphere in L3 larvae.

(Liu et al., 2015)

Marker for

Reflects expression of

(Liu et al., 2015)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

600601

w^*; PBac{Clk-V5-GFP}VK00018; Clk^out

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Clk-GFP

(Wang et al., 2025, Lv et al., 2024, Liu et al., 2015)

Clk-GFP-V5

(He et al., 2023)

PBac{Clk-V5-GFP}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (6)

Report Sections