FlyBase Allele Report: Dmel\Clk[Tag:V5,EGFP]

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Allele: Dmel\Clk^Tag:V5,EGFP

General Information

Symbol

Dmel\Clk^Tag:V5,EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0319048

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

PBac{Clk-V5-GFP}

Also Known As

Clk-GFP

Key Links

Transgenic product class

(Liu et al., 2015)

(Liu et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Liu et al., 2015)

(Liu et al., 2015)

Mutagen

in vitro construct

(Liu et al., 2015)

Progenitor genotype

Carried in construct

PBac{Clk-V5-GFP}

Cytology

Description

An approximately 15kb genomic fragment containing Clk. Tag:V5 and EGFP tags have been introduced at the C-terminal end of the Clk coding region.

(Liu et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Tagged with

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

larval DN1 neuron

Comment: 48h AEL

(Liu et al., 2015)

larval DN2 neuron

Comment: 48h AEL

(Liu et al., 2015)

larval s-LNv Pdf neuron

Comment: 48h AEL

(Liu et al., 2015)

second instar larval stage

larval DN1 neuron

Comment: 60h AEL

(Liu et al., 2015)

larval DN2 neuron

Comment: 60h AEL

(Liu et al., 2015)

larval LNd neuron

Comment: 72h AEL

(Liu et al., 2015)

larval s-LNv Pdf neuron

Comment: 60h AEL

(Liu et al., 2015)

third instar larval stage

adult LP neuron

(Liu et al., 2015)

larval s-LNv Pdf neuron

(Liu et al., 2015)

larval LNd neuron

(Liu et al., 2015)

larval DN period neuron

(Liu et al., 2015)

pupal stage P6 -- P7

adult LP neuron

(Liu et al., 2015)

adult s-LNv neuron

(Liu et al., 2015)

pupal DN period neuron

(Liu et al., 2015)

(Liu et al., 2015)

pharate adult stage P8 -- adult stage

adult LP neuron

(Liu et al., 2015)

(Liu et al., 2015)

adult s-LNv neuron

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

(Liu et al., 2015)

Additional Information

Statement

Reference

In all stages expression was analyzed at zeitgeber time 22 as well as at zeitgeber time 12 in the third instar larval stage. Clk^{T:SV5V5,T:AvicGFP-EGFP} is detected in around eight neurons per hemisphere in L1 and L2 larvae. This number increases to around 60 cells per hemisphere in L3 larvae.

(Liu et al., 2015)

Marker for

Reflects expression of

(Liu et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Clk^Tag:V5,EGFP partially rescues Clk^out

(Liu et al., 2015)

Comments

Clk^{T:SV5\V5,T:Avic\GFP-EGFP} significantly rescues circadian rhythms (94% of flies show rhythmicity with a 24.5hr period) in Clk^out/Clk^out flies under dark conditions.

(Liu et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

600601

w^*; PBac{Clk-V5-GFP}VK00018; Clk^out

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Clk^{T:SV5\V5,T:Avic\GFP-EGFP}

Clk^Tag:V5,EGFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections