FlyBase Allele Report: Dmel\Clk[out]

General Information

Symbol

Dmel\Clk^out

Species

D. melanogaster

Name

FlyBase ID

FBal0297400

Feature type

allele

Associated gene

Dmel\Clk

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Mahesh et al., 2014)

amorphic allele - molecular evidence

(Mahesh et al., 2014)

Mutagen

FLPase

(Mahesh et al., 2014)

piggyBac activity

(Mahesh et al., 2014)

Progenitor genotype

PBac{WH}Clk^f06808

(Mahesh et al., 2014)

PBac{WH}f03095

(Mahesh et al., 2014)

Cytology

Description

Clk^out is a 5398bp deletion generated through FLP-induced recombination between PBac{WH}Clk^f06808 and PBac{WH}f03095. The remaining PBac insertion has been excised. The Clk transcription start site and regulatory sequences that direct spatial and circadian expression have been deleted.

(Mahesh et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:7,770,259..7,775,657 [-]

(Mahesh et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^S258A.Tag:V5

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^{S476A.S482A.S487A.Tag:V5}

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^{S476A.S482A.Tag:V5}

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^S487A.Tag:V5

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^S525A.Tag:V5

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^S859A.Tag:V5

(Mahesh et al., 2014)

abnormal circadian rhythm, with Clk^S912A.Tag:V5

(Mahesh et al., 2014)

abnormal circadian rhythm (with Df(3L)BSC631)

(Mahesh et al., 2014)

abnormal circadian rhythm | adult stage

(He et al., 2023, Lee et al., 2016)

abnormal circadian rhythm | adult stage, with Clk^Tag:V5.WT

(Lee et al., 2016)

abnormal circadian rhythm | adult stage, with Clk^{Δ657-707.Tag:V5}

(Lee et al., 2016)

abnormal locomotor rhythm

(Liu et al., 2015)

abnormal locomotor rhythm, with Clk^15A.Tag:V5

(Lee et al., 2014)

abnormal starvation stress response | adult stage

(He et al., 2023)

abnormal temperature response, with Clk^15A.Tag:V5

(Lee et al., 2014)

decreased rate of feeding behavior | adult stage

(He et al., 2023)

female semi-fertile

(Wang et al., 2025)

female sterile, with Clk^UAS.L.Tag:V5, Scer\GAL4^NP1624

(Wang et al., 2025)

female sterile, with Clk^{UAS.S.Tag:FLAG}, Scer\GAL4^NP1624

(Wang et al., 2025)

male fertile

(Wang et al., 2025)

male fertile, with Clk^UAS.L.Tag:V5, Scer\GAL4^NP1624

(Wang et al., 2025)

male semi-fertile, with Clk^{UAS.S.Tag:FLAG}, Scer\GAL4^NP1624

(Wang et al., 2025)

viable

(Mahesh et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Clk^out homozygous adults reared under constant 25[o]C temperature and entrained to 12h:12h light:dark cycles show an increase in dark phase activity, show a decrease in morning startled activity, but do not show an obvious effect in evening startled activity, as compared to controls; upon transfer to constant darkness for 7 days, these individuals do not show circadian rhythmicity and almost do not show peaks of activities, as compared to controls.

Clk^out homozygotes bearing either Clk^Tag:V5.WT or Clk^{Δ657-707.Tag:V5} kept under constant darkness and subject to 12h:12h 24[o] C:29 [o]C temperature cycles exhibit bimodal peaks of activity with an increase in anticipatory activity before temperature transitions; these individuals maintain some rhythmicity and manifest 23.1h and 24.9h periods, respectively.

(Lee et al., 2016)

Clk^out/Clk^out flies lose circadian rhythms (0% show rhythmicity) under dark conditions.

(Liu et al., 2015)

Flies expressing Clk^15A.T:SV5\V5 in a Clk^out mutant background manifest generally weaker behavioral rhythms that are approximately 1.5 hours shorter compared to their wild type controls following exposure to entrainment conditions (12 hours light: 12 hours dark followed by several days in constant darkness). Clk^15A.T:SV5\V5, Clk^out mutant flies fail to entrain correctly to daily temperature cycles in the absence of light, but entrainment does occur in the presence of light.

(Lee et al., 2014)

Homozygous Clk^out mutant flies are viable and show no obvious developmental defects.

Flies expressing Clk^{S258A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, lengthening of the free-running period is seen compared to controls.

Flies expressing Clk^{S912A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, lengthening of the free-running period is seen compared to controls.

Flies expressing Clk^{S525A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, reduction in the free-running period is seen compared to controls.

Flies expressing Clk^{S859A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, reduction in the free-running period is seen compared to controls.

Flies expressing Clk^{S487A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, reduction in the free-running period is seen compared to controls.

Flies expressing Clk^{S476A.S482A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, reduction in the free-running period is seen compared to controls.

Flies expressing Clk^{S476A.S482A.S487A.T:SV5\V5} in a Clk^out mutant background exhibit locomotor activity rhythm defects. A small, but significant, reduction in the free-running period is seen compared to controls.

(Mahesh et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Clk^out has abnormal circadian rhythm | adult stage phenotype, suppressible by Gart^{10xUAS.Tag:V5}/Scer\GAL4^Myo31DF.PU/Scer\GAL80^nSyb.PS

(He et al., 2023)

Clk^out has decreased rate of feeding behavior | adult stage phenotype, suppressible by Gart^{10xUAS.Tag:V5}/Scer\GAL4^Myo31DF.PU/Scer\GAL80^nSyb.PS

(He et al., 2023)

Clk^out has abnormal starvation stress response | adult stage phenotype, suppressible | partially by Gart^{10xUAS.Tag:V5}/Scer\GAL4^repo

(He et al., 2023)

Clk^out has abnormal starvation stress response | adult stage phenotype, suppressible | partially by Gart^{10xUAS.Tag:V5}/Scer\GAL4^Cg.PU

(He et al., 2023)

Enhancer of

Statement

Reference

Clk^S859A.Tag:V5/Clk^out is an enhancer of abnormal locomotor rhythm phenotype of nmo^P1/Df(3L)Exel6279

(Mahesh et al., 2014)

NOT Enhancer of

Statement

Reference

Clk^out/Clk^Tag:V5.WT is a non-enhancer of abnormal locomotor rhythm phenotype of nmo^P1/Df(3L)Exel6279

(Mahesh et al., 2014)

NOT Suppressor of

Statement

Reference

Clk^out/Clk^Tag:V5.WT is a non-suppressor of abnormal locomotor rhythm phenotype of nmo^P1/Df(3L)Exel6279

(Mahesh et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Clk^T:SV5\V5.WT (in a Clk^out mutant background) does not alter the locomotor rhythm defects seen in nmo^P1/Df(3L)Exel6279.

Expression of Clk^{S859A.T:SV5\V5} (in a Clk^out mutant background) enhances the locomotor activity rhythm defects seen in nmo^P1/Df(3L)Exel6279 mutants.

(Mahesh et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Clk^out is rescued by Clk^Tag:V5.WT

(Lee et al., 2016)

Clk^out is rescued by Clk^Tag:V5.WT

(Lee et al., 2014)

Clk^out is rescued by Clk^Tag:V5.WT

(Mahesh et al., 2014)

Partially rescued by

Clk^out is partially rescued by Clk^Tag:V5,EGFP

(Liu et al., 2015)

Not rescued by

Clk^out is not rescued by Clk^{Δ657-707.Tag:V5}

(Lee et al., 2016)

Comments

The photo-period circadian rhythmicity defects observed in Clk^out homozygous adults are rescued by Clk^Tag:V5.WT but not by Clk^{Δ657-707.Tag:V5}.

(Lee et al., 2016)

Clk^{T:SV5\V5,T:Avic\GFP-EGFP} significantly rescues circadian rhythms (94% of flies show rhythmicity with a 24.5hr period) in Clk^out/Clk^out flies under dark conditions.

(Liu et al., 2015)

Expression of Clk^T:SV5\V5.WT rescues the behavioral rhythm defects seen in Clk^out following following exposure to entrainment conditions (12 hours light: 12 hours dark followed by several days in constant darkness). A similar rescue is seen at 18, 25 and 29[o]C. The failure of Clk^out mutant flies to entrain to daily temperature cycles in the absence of light is also rescued in these flies.

Expression of Clk^15A.T:SV5\V5 partially rescues the behavioral rhythm defects seen in Clk^out following exposure to entrainment conditions (12 hours light: 12 hours dark followed by several days in constant darkness). A similar rescue is seen at 18, 25 and 29[o]C.

(Lee et al., 2014)

Expression of Clk^T:SV5\V5.WT rescues the locomotor activity rhythm defects in >90% of Clk^out flies with periods of ~24.2 hours.

(Mahesh et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

56754

w^*; Clk^out

600601

w^*; PBac{Clk-V5-GFP}VK00018; Clk^out

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Clk^OUT

(Giesecke et al., 2023, Andreazza et al., 2015)

Clk^out

(Wang et al., 2025, Elya et al., 2023, He et al., 2023, Gunawardhana et al., 2021, Mahesh et al., 2020, Cho et al., 2019, Liu et al., 2019, Agrawal et al., 2017, Gunawardhana and Hardin, 2017, Lee et al., 2016, Liu et al., 2015, Lee et al., 2014)

clk^out

(Cai et al., 2024, Lv et al., 2024, Meyerhof et al., 2024, Tabuloc et al., 2023, Cai et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (20)

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