FB2026_03 , released September 17, 2026
Recombinant Construct: P{knon-GFPmut3}
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General Information
Symbol
P{knon-GFPmut3}
FlyBase ID
FBtp0117024
Feature type
transgenic_transposable_element
Component Allele(s)
knonGFP(mut3star)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele knonGFP(mut3star)
Transgenic product class
wild_type
(Sawyer et al., 2017)
Component
Use(s)
Regulatory region(s)
knon
Encoded product / tool
knon
Tagged with
GFP(mut3star)
Description

The knon coding region is expressed under the control of approximately 1.8kb of upstream regulatory sequence. A GFP(mut3star) tag has been introduced at the C-terminal end of the coding region.

(Sawyer et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis
primary spermatocyte
(Sawyer et al., 2017)
secondary spermatocyte
(Sawyer et al., 2017)
spermatid cyst
(Sawyer et al., 2017)
spermatozoon
(Sawyer et al., 2017)
Additional Information
Statement
Reference

Tagged protein is detected starting in spermatocytes during chromatin condensation and continuing through different stages of spermatogenesis. It colocalizes with mitochondria and persists in mitichondrial derivatives during and after mitochondrial elongation in elongated spermatid cysts. It is also detected in mature sperm.

(Sawyer et al., 2017)
 
Marker for
Reflects expression of
knon
(Sawyer et al., 2017)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{knon-GFPmut3}
(FlyBase, 1992-)
knon-GFP
(Sawyer et al., 2017)
Secondary FlyBase IDs
    References (3)