FB2026_02 , released June 18, 2026
Allele: Dmel\knonGFP(mut3star)
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General Information
Symbol
Dmel\knonGFP(mut3star)
Species
D. melanogaster
Name
FlyBase ID
FBal0326184
Feature type
allele
Associated gene
Dmel\knon
Associated Insertion(s)
Carried in Construct
P{knon-GFPmut3}
Key Links
Transgenic product class
wild_type
(Sawyer et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sawyer et al., 2017)
Mutagen
in vitro construct
(Sawyer et al., 2017)
Progenitor genotype
Carried in construct
P{knon-GFPmut3}
Cytology
Description

The knon coding region is expressed under the control of approximately 1.8kb of upstream regulatory sequence. A GFP(mut3star) tag has been introduced at the C-terminal end of the coding region.

(Sawyer et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
knon
Encoded product / tool
knon
Tagged with
GFP(mut3star)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      spermatogenesis
      primary spermatocyte
      (Sawyer et al., 2017)
      secondary spermatocyte
      (Sawyer et al., 2017)
      spermatid cyst
      (Sawyer et al., 2017)
      spermatozoon
      (Sawyer et al., 2017)
      Additional Information
      Statement
      Reference

      Tagged protein is detected starting in spermatocytes during chromatin condensation and continuing through different stages of spermatogenesis. It colocalizes with mitochondria and persists in mitichondrial derivatives during and after mitochondrial elongation in elongated spermatid cysts. It is also detected in mature sperm.

      (Sawyer et al., 2017)
       
      Marker for
      Reflects expression of
      knon
      (Sawyer et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      knonGFP(mut3star) rescues knon1400/Df(2R)7E

      (Sawyer et al., 2017)
      Comments

      The male sterility as well as the defective mitochondrial shaping and elongation during spermatogenesis observed in knon1400;Df(2R)7E is rescued by combination with knonT:Avic\GFP-mut3star.

      (Sawyer et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      knonGFP(mut3star)
      knonT:Avic\GFP-mut3star
      Name Synonyms
      Secondary FlyBase IDs
        References (1)