FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{lexAop-UAS-GrabFP.B.Ext.TagBFP}
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General Information
Symbol
M{lexAop-UAS-GrabFP.B.Ext.TagBFP}
FlyBase ID
FBtp0117302
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

lexAop and UAS regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that encodes an basolaterally localized nanobody-based GFP trap for extracellular proteins. The fusion protein contains the nrv1 coding sequence (from the LD02379 cDNA clone) with a TagBFP fluorescent tag inserted near the N-terminal end (between the first and second exon). In addition, the GFP-binding VHH(GFP4) nanobody has been inserted at the C-terminal end of the nrv1 coding sequence. The VHH(GFP4) nanobody faces the extracellular space. The lexAop and UAS regulatory sequences are flanked by loxP and lox2272 sites respectively (meaning that the P1cre recombinase can be used to excise one of the enhancers in a mutually exclusive manner if desired).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
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Phenotypic Data
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Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
GrabFP-BExt - pUASTLOTattB_vhhGFP4::Nrv1::TagBFP
LOP/UAS-GrabFP-BExt
M{lexAop-UAS-GrabFP.B.Ext.TagBFP}
UAS-GrabFPExt.Basolateral-mCherry
nrv-Morphotrap
Secondary FlyBase IDs
    References (6)