FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-GrabFP.B.Ext.TagBFP}
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General Information
Symbol
M{UAS-GrabFP.B.Ext.TagBFP}
FlyBase ID
FBtp0117307
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele nrv1UAS.GrabFP.B.Ext.TagBFP
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

UAS regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that encodes an basolaterally localized nanobody-based GFP trap for extracellular proteins. The fusion protein contains the nrv1 coding sequence (from the LD02379 cDNA clone) with a TagBFP fluorescent tag inserted near the N-terminal end (between the first and second exon). In addition, the GFP-binding VHH(GFP4) nanobody has been inserted at the C-terminal end of the nrv1 coding sequence. The VHH(GFP4) nanobody faces the extracellular space. (Generated by P1cre-mediated excision of the lexAop regulatory sequences present in nrv1lexAop.UAS.GrabFP.B.Ext.TagBFP).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-GrabFP.B.Ext.TagBFP}
UAS-GrabFP-BExt
Secondary FlyBase IDs
    References (3)