The full-length (1668bp) nerfin-1 3'UTR has been inserted into a Not-I site within the SV40 3'UTR of Avic\GFPEGFP.nerfin-1.SV40.Tag:NLS(tra), 14bp 3' of the EGFP ORF translation stop codon.
AvicGFPEGFP.nerfin-1.iA.Tag:NLS(tra) is present in differentiated elav positive medulla neurons but absent from pros positive ganglion mother cells.