FlyBase Recombinant Construct Report: P{UASp-CIBN-pmEGFP}

UASp regulatory sequences drive expression of the CIBN(ΔNLS) heterodimerization sequence, tagged at the C-terminal end with EGFP followed by a plasma membrane localization tag (Tag:PM(hKRAS)). CIBN(ΔNLS) heterodimerizes with the N-terminal photolyase homology region (PHR) of the Arabidopsis thaliana CRY2 gene upon exposure to blue light, and thus the expressed protein can be used to recruit any protein tagged with a compatible heterodimerization sequence (containing the CRY2 PHR domain) to the plasma membrane in a light-dependent manner.

(Guglielmi et al., 2015)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Atha\CIB1^{CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CIBN::pmGFP

(Deneke et al., 2019)

P[w+, UASp>CIBN::pmGFP]

(Krueger et al., 2019, Izquierdo et al., 2018, Guglielmi et al., 2015)

P{UASp-CIBN-pmEGFP}

(FlyBase, 1992-)

UASp-CIBN-CAAX

(Zhou et al., 2022)

UASp-CIBN-pm-GFP

(Guo et al., 2022)

UASp-CIBN::pmGFP

(Jacobs et al., 2025, Mitchell et al., 2022, Bhide et al., 2021)

UASp-pmGFP-CIBN

(Eritano et al., 2020)

UASp>CIBN-pmGFP

(Countryman et al., 2025, Herrera-Perez et al., 2021)

UASp>CIBN::pmGFP

(Godeau et al., 2025, Krueger et al., 2018)

Secondary FlyBase IDs

References (17)

Report Sections

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