FB2026_02 , released June 18, 2026
Recombinant Construct: P{FLAG-pnut.T509E.S517E}
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General Information
Symbol
P{FLAG-pnut.T509E.S517E}
FlyBase ID
FBtp0126857
Feature type
transgenic_transposable_element
Component Allele(s)
pnutT509E.S517E.Tag:FLAG
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele pnutT509E.S517E.Tag:FLAG
Transgenic product class
missense_variant
(Akhmetova et al., 2018)
polypeptide_post_translational_processing_variant
(Akhmetova et al., 2018)
Component
Use(s)
Regulatory region(s)
pnut
Encoded product / tool
pnut
Tagged with
Tag:FLAG
Description

The potential native promoter of pnut (1.7kb upstream of the translation start site) drives expression of pnut cDNA sequence that carries mutations to mimic constitutive phosphorylation (amino acid replacements T509E and S517E). The open reading frame is tagged at the N-terminal end with Tag:FLAG.

(Akhmetova et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
FLAG-pnut-T509E/S517E
(Akhmetova et al., 2018)
P{FLAG-pnut.T509E.S517E}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)