FlyBase curator comment: TI{GT-GAL4} represents a DNA segment which contains a splice acceptor site, the GAL4 driver coding sequence and typically a 3' polyadenylation signal and which has been inserted into the genome via any technique (such as CRISPR/Cas9 or other targeted mutagenesis) that results in the inserted DNA not being flanked by transposable element ends. The presence of the splice acceptor site means that the TI{GT-GAL4} sequence can act as a gene trap element when inserted into an intron of an endogenous locus, resulting in expression of the GAL4 driver under the regulatory sequences of the trapped gene. When the sequence contains a 3' polyadenylation signal, the insertion of TI{GT-GAL4} may also be mutagenic, truncating the endogenous transcript and potentially disrupting the function of the trapped gene. Other sequences may be present in the inserted DNA and the precise sequence inserted may differ for different insertions of the construct. For details of what is inserted into the genome for an individual insertion of TI{GT-GAL4}, see the description in the insertion report and in any allele reports associated with that insertion.