FB2026_03 , released September 17, 2026
Recombinant Construct: P{RLY}
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General Information
Symbol
P{RLY}
FlyBase ID
FBtp0131264
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgenic construct that is designed to test for chromosomal position effects (CPE) when inserted in the genome. It comprises 3'-to-5': an attR site, an FRT cassette containing the M2 insulator (a dimer of the 215bp M fragment from the scs' insulator, including the high affinity BEAF-32 protein binding site), a w+mC minigene, a 990bp scs insulator, an attL site, and a loxP cassette containing the y+mDint2 marker. The M2 insulator can be excised using FLP recombinase to test for CPE at a particular genome insertion site, allowing insertion sites with the desired CPE properties to be identified. The sequence between the attL and attR site can then be excised using phiC31:Dint(KEE) disintegrase, regenerating a functional attP 'landing' site that is marked with a loxP cassette containing y+mDint2.

(Maharjan et al., 2018)
Construct components
Component
Use(s)
Also carries
FRT
loxP
Marker(s)
y+mDint2
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
P{attP.loxP(y+)}
(Maharjan et al., 2018)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{RLY}
(Maharjan et al., 2018, FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)