ScerGAL4AstA.2.74 drives expression in two to three AstA positive interneurons with somata in the posterior lateral protocerebrum that also express Pdfr. These send a primary neurite dorsally, just anterior of the calyx, which trifurcate and then arborize throughout the whole superior lateral, superior intermediate and superior medial protocerebrum. These arborizations extend from the height of the fan-shaped body to just anterior of the calyx. Expression is seen in two to four AstA negative cells per hemisphere with somata in the cell body rind of lateral neuropils close to the lateral horn, as well as faintly in a small number of AstA postive cells in the medulla. In the adult abdominal neuromere, expression is seen in three pairs of AstA positive cells within the dorsolateral part of the posterior abdominal neuromere, these send neurites via the abdominal nerve trunk to innervate the hindgut and posterior-most midgut, including the adult rectal valve and adult pylorus and send processes to innervate the rectum which also part extend through the muscle layer. Expression is also seen in AstA positive nerves innervating the wings and halteres, and AstA positive adult enteroendocrine cells
